AutoQuant X Deconvolution & 3D Visualization Imaging Software from Meyer Instruments, Inc.

1304 Langham Creek, Suite 235
Houston, TX 77084
Phone: 281-579-0342
Fax: 281-579-1551

AutoQuant X
Deconvolution & 3D Visualization
Imaging Software

AutoDeblur

All microscopes are limited by the laws of physics, and these laws state that when light passes through a medium, that light will bend. This is one of the most common causes of haze and blur in microscopy images. Deconvolution can correct this problem, not only removing the haze and blur, but restoring vital detail to datasets.

AutoQuant X is the most advanced image deconvolution and 3D visualization software available today!

AutoDeblur offers the most powerful Deconvolution tools available, including AutoQuant X's proprietary Blind Deconvolution . The Blind Deconvolution algorithm is both iterative and constrained, yet unlike other deconvolution products, it does not require the manual calibration and measurement of the point spread function (PSF). Instead, it constructs the PSF directly from the collected data set. AutoDeblur works with Widefield Epi-Fluorescence, DIC, Transmitted-Light Brightfield and most Confocal microscopes including Two-Photon and Spinning Disk.

In addition to the full compliment of algorithms, AutoDeblur offers several other advantages as well. For starters, it is a multiDimensional platform, capable of handling 3D time-series datasets. Instead of loading each time point one at a time, and processing them through a batch process, they can be loaded and processed as a single dataset.

Another advantage of AutoDeblur is the ability to load a nearly limitless number of channels, freeing you from the constraints of the world of RGB. Advanced researchers are more and more often using multiple lasers and dyes, and AutoQuant X steps up to let their image processing be limited by their hardware rather than their software. Along the same lines, users also have full control over the display of each channel. You can choose from the lists of custom colors, wavelengths, dyes, or color mapping to best display your dataset.

	On the hardware side of the application, AutoDeblur is also multiThreaded. This gives you the ability to run concurrent processes. To take it a step further, dual processors can be utilized to their maximum potential by running CPU intensive processes concurrently on each one (such as deconvolution).

Mouse over image to see animation of before and after AutoDeblur

AutoVisualize

The goal of creating, enhancing, and saving images is to see, analyze and measure them, from all angles. AutoVisualizewill let you do just that. With AutoQuant X's 5D Viewer, time-series datasets can be rotated through time to any angle, an orthogonal slice can be moved through the dataset, you can create movies showing the full rotation of the dataset, multiple projections are available for dynamic display of your dataset.

Side by side before and after comparison has never been easier than now with AutoQuant X’s multiPreviewer capability. Multiple concurrent 5D Viewers can be opened and synchronized for striking comparisons of data. Use the Movie Maker feature to create an .avi file of a movie as your data rotates through time, and use it in a PowerPoint presentation, or post it to the web.

Additionally, deconvolving and viewing the dataset are only part of the process; once these are done, analysis is the next step. With AutoVisualize, you can measure distances between objects, measure the surface area and volume of objects, and calculate statistics on the dataset.

Colocalization

Multi-channel images often have sections where two or more of the dyes overlap. AutoQuant X's Colocalization module can identify these areas, display them, and generate statistics on user-selected regions of interest.

An easy to use interface lets you load in two channels of a multi-channel dataset, create an intensity mask in which to display the colocalized areas, as well as generate a new "colocalized" dataset, displaying only the areas with Colocalization.

FRET

Created for researchers focusing on protein-protein interactions, our FRET module incorporates the two most commonly accepted algorithms: Elangovan and Periasamy, and Gordon and Herman, and adds our own proprietary algorithm as well. All three algorithms correct Cross Talk, but AutoQuant X's proprietary algorithm goes one step further. Where other algorithms make assumptions about the Cross Talk, our Maximum Likelihood Estimation algorithm mathematically solves the Cross Talk, allowing for a much more accurate analysis of the images.

FRET X also includes several helpful preprocessing tools to turn your images into precisely analyzed statistics. The Channel to Channel alignment tool corrects for shifts between channels, shifts that would otherwise corrupt your analyses. The Background Subtraction tool eliminates artifacts that can compromise the results of your analyses.

Once the datasets have been processed with the desired algorithm, easily create as many regions of interest as you desire, then run statistical analyses on those ROI’s, and export the results to a spreadsheet.

For added ease of use, FRET X is multiThreaded, meaning more than one process can be running at a time. Also, there is an autoLoad feature, so once you have loaded, and made filter set associations for a set of data, those are saved, to make reprocessing them much easier and less time-consuming.

Image Alignment

A common problem during 3D image acquisition is misalignment between the slices due to stage vibration, filter cube, or a host of any other causes. AutoQuant X's Image Alignment module is the cure for this ill. With powerful and accurate algorithms for slice to slice alignment as well as channel to channel alignment, AutoQuant X can correct virtually any misalignment issue. Our Image Alignment feature handles problems from vertical to horizontal shift, warping, as well as rotation.

Object Counting and Tracking

The ultimate in time-series image analysis, our Object Counting and Tracking module has the ability to count a nearly infinite number of objects. Our platform is multiDimensional compatible, and can load and process 3D time-series datasets, making it the ultimate tool for counting and tracking 3D objects through time. Counting and Tracking X has powerful and intuitive preprocessing tools to give complete control over the objects to be counted and tracked.

Once the objects have been counted, the objects can then be tracked through the time series. Easy to follow tracking lines show where the object has moved through time, for a vivid graphic depiction of the objects’ activities.

Finally, properties such as the size, circularity, volume, speed, acceleration, distance traveled between time-points and much more can be calculated and exported to a spreadsheet for later analysis.

Ratiometrics

This module is tailored for intercellular ionic imaging. Designed for researchers who study the effect of changing the environment of a sample by comparing the same sample with differing concentrations of calcium, or changing the pH, Ratiometrics X fills the bill.

Ratiometrics X employs the Grynkiewicz Equation for Ion Concentration and produces accurate results with visually-emphasized color mapping.

Built in pre and postprocessing steps such as Automatic Alignment, Remove Spot Noise and Gaussian Smoothing make for a cleaner resultant image with less steps for the user.

Once the preprocessing has been done, Ratiometrics X delivers concise statistics, and is easily exported into an xls file. Select specific areas to analyze by creating regions of interest, fully defined by the user. Get the results you need from the areas you want.

Product Name	What it includes	Product Description
AutoDeblur 2D	2D Blind Deconvolution	A constrained-iterative approach to deconvolve a single 2D image or stack of 2D images, such as a time-series
AutoDeblur Silver WF	No Neighbors/Nearest Neighbors/Inverse Filter/2D	2D deconvolution + NN/InvFilter for Widefield microscopes
AutoDeblur Silver CF	1D/2D/XzYz	2D deconvolution + Axial Blind for Confocal microscopes
AutoDeblur Silver CWF	All of the Above	Combined Widefield and Confocal applications
AutoDeblur Gold WF	3D Blind, 2D Blind, Inverse Filter, No/Nearest Neighbors	3D deconvolution for Widefield microscopes
AutoDeblur Gold CF	3D Blind, 2D Blind, 1D, XzYz	3D deconvolution for Confocal microscopes
AutoDeblur Gold CWF	All of the above	Combined Widefield and Confocal applications
AutoVisualize	Visualization/Morphometry/Base Platform for Advanced Image Analysis Modules	Visualization, Measurements, Data Rotation, 3D movies
Non-Blind Deconvolution Plug-In	Imports PSF - for Gold WF, CF, CWF	Optional feature for importing theoretical or measured PSF
Image/Color Alignment Plug-In	Aligns slices in XY/Use with AutoDeblur or AutoVisualize	Optional feature used to correct for motion or vibration between slices during acquisition
FRET/Ratiometrics	Advanced Image Analysis module	For use with the AutoVisualize Platform
Object Counting/Tracking	Advanced Image Analysis module	For use with the AutoVisualiize Platform
Colocalization	Advanced Image Analysis module	For use with the Autovisualize Platform
AutoAnalyze	FRET/Ratiomtrics, Object Counting/Tracking and Colocalization	For use with the Autovisualize Platform
AutoDeblur Maint. Agreement	Includes upgrades for one year	Includes upgrades for one year
AutoVisualize Maint. Agreement	Includes upgrades for one year	Includes upgrades for one year
AQI "Combo" WF	Combination Savings Package	AutoDeblur Gold WF, AutoVisualize, Non-Blind and Alignment Plug-ins
AQI "Combo" CF	Combination Savings Package	AutoDeblur Gold CF, AutoVisualize, Non-Blind and Alignment Plug-ins
AQI "Combo" CWF	Combination Savings Package	AutoDeblur Gold CWF, AutoVisualize, Non-Blind and Alignment Plug-ins

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(Created 04-Jan-2005 - AAK)
(Modified 27-May-2009 - AAK)