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Resolution's Digital Volumetric Imaging (DVI) is a radical advance in digital microscopy that for the first time permits routine generation of high-fidelity three-dimensional images of tissue and other materials. For over a century, research microscopists have sought a means to produce three-dimensional views of the materials they studied. However, tissue-based microscopy relies on histologic technique, an assembly line manufacturing process dedicated to the fabrication of one product, the glass microscope slide. This dependence seriously limits the quantity and quality of data that can be obtained from materials under examination.
Resolution's technology realigns the traditional microscopy paradigm by eliminating the requirement to fabricate a glass slide before producing an image, thereby disposing of the inefficient labor component of tissue-based microscopy. This conceptual breakthrough enables histopathology to progress to a new level, where the processing of information, rather than the manufacture of a product becomes the focus of attention.
The advantages of the DVI System flow from a central, unique feature: while conventional histopathology relies on the creation of glass slides on which are mounted thin tissue sections cut from a tissue block, the DVI System instead captures high-resolution virtual sections directly from the block's surface. This process, also called surface imaging microscopy, permits the collection of a much greater amount of information from each sample by automating the collection of large numbers of serial sections. In essence, the DVI System images a tissue section before it is cut from a sample, rather than after, eliminating the need to produce histologic sections. This breakthrough makes possible automated serial sectioning of entire samples, and the production of unprecedented three-dimensional digital representations.
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Unlike conventional histopathologic technique, which examines a few cuts taken more or less at random through a sample, the DVI System concept gives microscopists access to thousands of precisely registered, high fidelity serial sections, comprising an accurate digital replica of the tissue. The user has full control over the selection of two-dimensional images from this three-dimensional data. In addition, this revolutionary technology can yield three-dimensional digital reproductions of tissue architecture in unprecedented detail, and can do so on far larger volumes of tissue than can be addressed by other microscopy methods.
The potential for a dramatic increase in the amount of information extracted from materials gives the Resolution's DVI System a powerful advantage over standard techniques.
The high-fidelity datasets produced by Digital Volumetric Imaging (DVI) technology are visualized with RESView, a proprietary software package developed by Resolution. RESView allows you to visualize and analyze digitized two- and three-dimensional information from tissue and other materials. The RESView Workstation includes the RESView software, a specially configured Windows NT™ workstation and example datasets.
 Trabecular Bone with Fluorescent Label |
 Cross Section of a Mouse Tail |
 Skin |
 Labyrinth |
 Maize Root Tip |
 Cartilage |
 Intestine |
 Sheep Lung Blood Vessel |
 VELCRO® |
 Micro-fiber Textile |
 Coated Magazine Cover |
 MEMS Device, Micro Machine Part |
 Hand-made Paper |
 Metal Foam |
 Medical Grade Gauze |
All sample images are Copyrighted © 2001 Resolution Sciences Corporation. All Rights Reserved.
