Leica Application Suite

The new Leica Application Suite integrates Leica automated microscopes, digital cameras and software into one common micro-imaging environment to provide an easy to use and consistent platform. Leica Application Suite solves and accelerates routine and research analysis.

The rich image processing functions make it suitable for a diverse range of imaging tasks such as visualization, enhancement, measurements, and documentation.

Additional modules can be added to further enhance the powerful functionality of LAS such as Image Overlay, MultiFocus, MultiTime, MultiStep, Extended Annotation, Interactive Measurement, and Reticule requirements.

Current Available Modules Listed Below

Leica Application Suite: Montage

Leica LAS Montage allows extended depth of field (EDF) and visually enhances focus for the most demanding imaging conditions.

The LAS Montage module works with Leica microscopes and digital cameras to automatically capture a stack of images at different focus positions.

These images are then combined into a single Extended Depth of Focus (EDF) image that shows a single image with all in focus parts of the stack.

Z-stack gallery allows a specific image to be quickly located and easily viewed. In conjunction with a microscope or stereomicroscope equipped with motorized focus the acquisition is completely automatic. Manual operation is also possible.

Multiple EDF algorithms allows for faster, higher quality extended depth of focus images. User editing controls provide the best in final image quality.

Leica Application Suite: Image Overlay

Leica LAS Image Overlay creates overlay images from multiple-wavelengths for sublime visualization.

The LAS Image Overlay module acquires enhances and documents multiple wavelengths using fluorescence microscopy images.

Constructed for the visualization, enhancement and digital documentation of fluorescence microscope images, it is versatile, making it suitable for users in bioscience, medical and pharmacological laboratories.

Designed for multi-channel imaging, the system performs tasks in a simple, automatic manner.

Leica Application Suite: Interactive Measurement

Leica LAS Interactive Measurement allows an extensive range of measurement types with uncompromised convenience.

The LAS Interactive Measurement module, fully integrated with Leica microscopes and digital cameras, allows the user to trace around objects with adjustable tracing lines, identify areas of significance and derive measurement parameters such as length, area, angle or perimeter.

Measurements can be applied to one image or multiple images to determine statistical trends. Each measurement can be labeled with a parameter name, sequence number, value and comment for quick, easy analysis.

Leica Application Suite: Extended Annotation

Leica LAS Extended Annotation allows images to be embellished with text, graphics and measurements to clearly communicate your ideas.

The LAS Extended Annotation module integrated with Leica Microsystems’ automated microscopes, digital cameras and software, allows graphic elements and text to be added to images.

Important features of the image can be highlighted using various fonts, colors and shapes.

Annotations may be added as a separate layer to the original file so the original remains intact or copied as a group and used as a template. The annotations increase and decrease proportionally with the selected image.

Leica Application Suite: MultiTime Movie Recorder

Leica LAS MultiTime captures time-lapse and movie images.

The LAS MultiTime module integrated with Leica microscopes and digital cameras automatically captures images at set time intervals such as seconds, minutes, hours or days.

And, for a specific time period, select the number of images may be acquired. Images are stored on the hard drive at the specified times and may be easily and quickly retrieved as an individual image or as an AVI movie file.

Alternatively capture the live image and stream directly to the hard dirve for later replay as a Movie.

Leica Application Suite: Reticule

Leica LAS Reticule allows your own reticules shown on the live image for easy comparison.

The LAS Reticule provides electronic overlays or permanent records of customized reticules on real-time images during routine inspections for point counting measurements and comparisons.

A range of reticule styles, such as lines, shapes, texts and images; cross hairs; and counting grids can be easily displayed, adjusted, stored and recalled.

Leica Application Suite: Multistep

Leica LAS MultiStep easily captures images larger than the microscope field of view.

The LAS Multistep module integrated with Leica microscopes and digital cameras automatically captures and easily stores large two dimensional images created from multiple tiled images.

Users may easily retrieve a single image for individual detailed evaluation or mosaic of multiple images for use with LAS Analysis module.

Leica Application Suite: Multifocus

Leica LAS Multifocus creates a single sharp image, effectively extending the depth of focus of the microscope or stereomicroscope.

The LAS Multifocus module works with Leica microscopes and digital cameras to automatically capture a stack of images at different focus positions.

These images are then combined into a single Extended Depth of Focus image that shows in a single image all in-focus parts of the stack.

Z-stack gallery allows a specific image to be quickly located and easily viewed. In conjunction with a microscope or stereomicroscope equipped with motorized focus the acquisition is completely automatic. Manual operation is also possible.

Multiple EDF algorithms allows for faster, higher quality extended depth of focus images. User editing controls provide the best in final image quality.

Leica AF6000 E

The Leica AF6000 is a fully integrated system for advanced fluorescence imaging providing solutions that evolve with your changing research requirements. From overlaying multi-channel images to acquiring three dimensional and time lapse data, a wealth of solutions are included as standard for image documentation, quantification, enhancement and analysis.

Designed to completely harmonize microscope, camera and application, the modular AF6000 system is available for both upright and inverted microscopes. Additional application modules can be added to extend the functionality for deconvolution and 3D visualization.

Leica AF6000

The Leica AF6000 is a fully integrated system for advanced fluorescence imaging providing solutions that evolve with your changing research requirements. From overlaying multi-channel images to acquiring three dimensional and time lapse data, a wealth of solutions are included as standard for image documentation, quantification, enhancement and analysis.

Designed to completely harmonize microscope, camera and application, the modular AF6000 system is available for both upright and inverted microscopes. Additional application modules can be added to extend the functionality for deconvolution and 3D visualization.

Leica AF6000 LX

Leica Microsystems introduces an integrated system for advanced widefield fluorescence imaging and analysis - the new Leica AF6000 LX. The highly integrated workstation enables researchers to study the processes of life. Imaging fast cell dynamics or 4D experiments over several days can easily be performed. Stable for long term experiments, it keeps the cells alive and healthy.

Camera, sample position and spectral selection are harmonized to enable ultra-fast imaging in real time. The new Super-Z Focus permits objective independent high precision z-positioning. The dedicated HCX PL APO 63x/1.3 Glyc Corr. live cell objective ensures crisp and sharp images even under physiological conditions. The rich choice of live cell accessories completes this integrated workstation.

Leica AF6000 E

Leica AF6000 E research software makes high-quality fluorescence image acquisition and documentation easy.

Leica AF6000 E enhances the performance of a wide range of manual and automated microscopes and cameras from Leica Microsystems. Ideal for everyday fluorescence imaging applications such as biological and cytomorphological examinations, the software provides professional documentation of research results.

Truly flexible, the software can also be configured with the Leica M205 FA automated stereofluorescence microscope.

Leica AF6500

The Leica AF6500 fluorescence microscope system is ideal for ultra-fast 2D time-lapse experiments and ratio imaging.

Leica Microsystems’ upright and inverted microscopes are the ideal system platforms. The ratio imaging function measures concentrations of second messenger substances such as Ca2+ at high-resolution over long time periods.

The system is also ideal for observing cellular processes such as exocytosis, transport processes within the cytoskeleton, and membrane fusions.

Leica AF7000

This advanced fluorescence imaging solution for highly demanding applications offers real-time, easy-to-use control for fast 3D time-lapse experiments, TIRF, Fura2, FRET SE, deconvolution, and peripheral triggering via four channels.

The versatile, fully integrated Leica AF7000 offers harmony between hardware and software and combines the ultimate speed, reliability, and facilities for experiment design, analysis, and operational convenience.

Providing real-time control of hardware for complex live cell studies, the researcher can observe live cells using TIRF.

Leica MM AF

Bio imaging techniques contribute to a growing number of scientific breakthroughs. The Leica MM AF Imaging System powered by MetaMorph® plays a large role in this revolution. With its image acquisition, processing and analysis capabilities, and complete set of tools for automation, Leica MM AF opens the door for new insights into cellular function.

Its flexibility and versatility make it a powerful system for multi-dimensional image acquisition and 3D reconstruction, and for measurements such as morphometry, colocalization and motility.