Leica Application Suite

The new Leica Application Suite integrates Leica automated microscopes, digital cameras and software into one common micro-imaging environment to provide an easy to use and consistent platform. Leica Application Suite solves and accelerates routine and research analysis.

The rich image processing functions make it suitable for a diverse range of imaging tasks such as visualization, enhancement, measurements, and documentation.

Additional modules can be added to further enhance the powerful functionality of LAS such as Image Overlay, MultiFocus, MultiTime, MultiStep, Extended Annotation, Interactive Measurement, and Reticule requirements.

Current Available Modules Listed Below

Leica Application Suite: Montage

Leica LAS Montage allows extended depth of field (EDF) and visually enhances focus for the most demanding imaging conditions.

The LAS Montage module works with Leica microscopes and digital cameras to automatically capture a stack of images at different focus positions.

These images are then combined into a single Extended Depth of Focus (EDF) image that shows a single image with all in focus parts of the stack.

Z-stack gallery allows a specific image to be quickly located and easily viewed. In conjunction with a microscope or stereomicroscope equipped with motorized focus the acquisition is completely automatic. Manual operation is also possible.

Multiple EDF algorithms allows for faster, higher quality extended depth of focus images. User editing controls provide the best in final image quality.

Leica Application Suite: Image Overlay

Leica LAS Image Overlay creates overlay images from multiple-wavelengths for sublime visualization.

The LAS Image Overlay module acquires enhances and documents multiple wavelengths using fluorescence microscopy images.

Constructed for the visualization, enhancement and digital documentation of fluorescence microscope images, it is versatile, making it suitable for users in bioscience, medical and pharmacological laboratories.

Designed for multi-channel imaging, the system performs tasks in a simple, automatic manner.

Leica Application Suite: Interactive Measurement

Leica LAS Interactive Measurement allows an extensive range of measurement types with uncompromised convenience.

The LAS Interactive Measurement module, fully integrated with Leica microscopes and digital cameras, allows the user to trace around objects with adjustable tracing lines, identify areas of significance and derive measurement parameters such as length, area, angle or perimeter.

Measurements can be applied to one image or multiple images to determine statistical trends. Each measurement can be labeled with a parameter name, sequence number, value and comment for quick, easy analysis.

Leica Application Suite: Extended Annotation

Leica LAS Extended Annotation allows images to be embellished with text, graphics and measurements to clearly communicate your ideas.

The LAS Extended Annotation module integrated with Leica Microsystems’ automated microscopes, digital cameras and software, allows graphic elements and text to be added to images.

Important features of the image can be highlighted using various fonts, colors and shapes.

Annotations may be added as a separate layer to the original file so the original remains intact or copied as a group and used as a template. The annotations increase and decrease proportionally with the selected image.

Leica Application Suite: MultiTime Movie Recorder

Leica LAS MultiTime captures time-lapse and movie images.

The LAS MultiTime module integrated with Leica microscopes and digital cameras automatically captures images at set time intervals such as seconds, minutes, hours or days.

And, for a specific time period, select the number of images may be acquired. Images are stored on the hard drive at the specified times and may be easily and quickly retrieved as an individual image or as an AVI movie file.

Alternatively capture the live image and stream directly to the hard dirve for later replay as a Movie.

Leica Application Suite: Reticule

Leica LAS Reticule allows your own reticules shown on the live image for easy comparison.

The LAS Reticule provides electronic overlays or permanent records of customized reticules on real-time images during routine inspections for point counting measurements and comparisons.

A range of reticule styles, such as lines, shapes, texts and images; cross hairs; and counting grids can be easily displayed, adjusted, stored and recalled.

Leica Application Suite: Multistep

Leica LAS MultiStep easily captures images larger than the microscope field of view.

The LAS Multistep module integrated with Leica microscopes and digital cameras automatically captures and easily stores large two dimensional images created from multiple tiled images.

Users may easily retrieve a single image for individual detailed evaluation or mosaic of multiple images for use with LAS Analysis module.

Leica Application Suite: Multifocus

Leica LAS Multifocus creates a single sharp image, effectively extending the depth of focus of the microscope or stereomicroscope.

The LAS Multifocus module works with Leica microscopes and digital cameras to automatically capture a stack of images at different focus positions.

These images are then combined into a single Extended Depth of Focus image that shows in a single image all in-focus parts of the stack.

Z-stack gallery allows a specific image to be quickly located and easily viewed. In conjunction with a microscope or stereomicroscope equipped with motorized focus the acquisition is completely automatic. Manual operation is also possible.

Multiple EDF algorithms allows for faster, higher quality extended depth of focus images. User editing controls provide the best in final image quality.

Leica AF6000

The Leica AF6000 provides all the necessary facilities for demanding fluorescence applications. This flexible, fully integrated system can be equipped with a large number of application packages and software modules.

The Leica AF6000 application spectrum ranges from general fluorescence work via multi-channel imaging to time-lapse experiments, multi-positioning and z-stacking. The system incorporates a wealth of standard features for image documentation, processing, quantification and analysis.

Optional modules for FRET, advanced time-lapse, channel unmixing, colocalization and various deconvolution techniques will extend the analysis capability.

Leica AF6000 E

The Leica AF6000 E is the ideal entry-level solution for widefield
fluorescence microscopy. It is particularly suited to general fluorescence imaging applications such as biological and cytomorphological examinations and for professional documentation of research results.

The system is truly flexible, as it can be teamed with both manual
and automated Leica Microsystems microscopes. In addition, the
Leica AF6000 E can be configured with the Leica M205 FA, Leica
Microsystems’ automated stereofluorescence microscope. The
system is compatible with many cameras in the Leica Microsystems
range, which benefit from full integration with the software.

As with all systems in the Leica Advanced Widefield System Line,
the Leica AF6000 E features an intuitive user interface and intelligent workflow guidance.

Leica AF6500

The Leica AF6500 is a high-speed fluorescence system that allows
real-time control of a camera and a filter wheel as required for
fast 2D time-lapse studies. Automated research microscopes,
both upright and inverted, from Leica Microsystems are the ideal
platforms for a Leica AF6500.

Use the ratio imaging function to measure concentrations of second messenger substances such as Ca2+ at resolution over long
periods of time. The system is also ideal for examinations of a
wide variety of cellular processes such as exocytosis, transport
processes within the cytoskeleton and membrane fusions.

Leica AF7000

The Leica AF7000 leaves nothing to be desired in widefield fluorescence imaging. This system combines the ultimate in speed, reliability, scope for experiment design, analysis facilities and operational convenience. Achieve excellent results – fast!

This extremely versatile and fully integrated system allows realtime control of all necessary hardware components for complex live cell studies, such as the camera, fine focus devices and up to four external filter wheels. In addition, it offers the possibility to observe live cells using TIRF. With the Leica AF7000 you are expertly equipped for the research tasks of today and tomorrow.

The Leica AF7000 effortlessly performs fast 3D time-lapse experiments. It is also fully capable of performing FRET SE, TIRF, as well as fast Fura2 measurements. Trigger signals can be transmitted to peripheral components via four channels. Vice versa, trigger signals can be received in up to four channels from external components and used to start experiments.

Leica MM AF

Bio imaging techniques contribute to a growing number of scientific breakthroughs. The Leica MM AF Imaging System powered by MetaMorph® plays a large role in this revolution. With its image acquisition, processing and analysis capabilities, and complete set of tools for automation, Leica MM AF opens the door for new insights into cellular function.

Its flexibility and versatility make it a powerful system for multi-dimensional image acquisition and 3D reconstruction, and for measurements such as morphometry, colocalization and motility.