Leica Structured Illumination is a wide-field imaging technique that uses the proven OptiGrid® module to produce confocal like images from ordinary research fluorescence microscopes. Designed to eliminate out of focus fluorescence blurring common to wide-field applications, the Leica Structured Illumination (SI) creates the perfect high resolution and high contrast image.

Fast and efficient 2D and 3D fluorescence images are obtained using a combination of imaging hardware, software, and a wide-field fluorescence microscope with a digital camera.

The same OptiGrid® module can be used in both upright and inverted Leica research microscopes.

Convenient and time saving, one optimized grid can be used for all objectives, 10x - 100x oil. No need to manually change grids and risk damage.

Automatic grid focusing produces high-resolution images at the press of a button. Both 0.63x and 1.0x C-mount adapters can be used with color and monochrome digital cameras to generate high quality images with the largest possible field of view.

Powerful software Leica MM AF makes setup and image acquisition easy. Both wide-field and (SI) images can be obtained. The Leica MM AF software suite also covers the following applications:

• Multidimensional imaging
• Image overlay
• Colocalization
• Morphometric measurements
• Intensity quantifications
• Time-lapse imaging
• Morphological filters
• Manual tracking