FLUORESCENCE APPS
Fluorescence APPs work on images and digital slides with immunofluorescent stains. Grayscale channel images have to be used. In the following, find a representative selection of StrataQuest Fluorescence APPs.
IF COEXPRESSION
The IF CoExpression APP provides single cell based co-expression analysis for multiple IF markers (the number is technically unlimited). It segments cells into nucleus, and/or perinuclear area and/or cytoplasm. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters which can be displayed in scattergrams and histograms and exported.
APP Category 1
IF COEXPRESSION+
The IF CoExpression+ APP provides single cell based co-expression analysis for multiple IF markers (the number is technically unlimited). It segments cells into nucleus, and/or perinuclear area and/or cytoplasm, as well as into membrane. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters which can be displayed in scattergrams and histograms and exported.
APP Category 2
IF META.CELLS
The IF Meta.Cells APP combines the detection of IF stained metastructures (e.g. Langerhans islets) with single cell detection (segments cells into nucleus, and/or perinuclear area and/or cytoplasm). Detected cells can be classified and visualized as being either within or outside of detected metastructures. Each detected area and segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters.
APP Category 2
IF DOTS
The IF Dots APP provides dot detection per cell within the cell compartments for up to four markers in a sample (e.g. FISH, RNA, oil droplets). Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters. Dot measurement parameters are provided per cell compartment (e.g. nucleus, cytoplasm) and include count, mean intensity, total dot area, and sum of intensity as well as area and intensity lists for all single dots.
APP Category 2
IF GRANULOMA
The IF Granuloma APP detects granulomas based on nuclear structure analysis and an adequate IF staining (e.g. CD11c, CD68). The number and area of Granulomas as well as their density is measured. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters.
APP Category 2
IF IMMUNE STATUS IN SITU
The IF Immune Status in Situ APPprovides phenotypic characterization of immune cells in reference to detected metastructures (e.g. tumors, glands) and measures the distance of detected cellular objects to the metastructure boundary (within and/or outside). Distance ranges can be defined. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters, as is the distance of each cell to the areas boundary.
APP Category 3
IF CYTOSKELETON
The IF Cytoskeleton APP detects cytoskeletal structures based on a specific stain. Used with other stains the cell cytoplasm can be detected. The number of cytoskeletal filaments inside of the cell, outside or on the cell membrane can be exported, as well as filament length and total filament area.
APP Category 3
IF NEURITE
The IF Neurite APP identifies neuronal cells and cell clusters and their neurites. It quantifies the number of neurites branching out from a specific neuron, identifies branch points and exports total neurite area, total neurite length, average neurite thickness, number of branch points, and number of end points.
APP Category 3
IF CELLULAR MICROENVIRONMENT
The IF Cellular Microenviroment APP allows to determine the cellular phenotype of specific IF stained cell populations and establishes their spatial relationship between each other, their neighbour cells/cell populations as well as the one with metastructures (e.g. blood vessels, tumors) in their vicinity. It is especially suited for proximity and infiltration analyses.
APP Category 4
IF LEISHMANIASIS
The IF Leishmaniasis APP detects intracellular Leishmania parasites and segments them in the detected host cells. The number of parasites per cell is determined and living and dead parasites can be distinguished (live/dead assays). The APP outputs the following data: 20 intensity, statistic and morphometric parameters for each segmented cell compartment per marker. Number, mean intensity, sum of intensity, and size of parasites.
APP Category 2
IF HI-PLEX 50
The IF Hi-Plex 50 APP combines and analyses images of the same tissue section IF stained/acquired for up to 50 times with different markers. The APP allows to detect the cellular phenotypes of specific IF stained cell populations. It segments cells into nucleus, and/or perinuclear area and/or cytoplasm. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters which can be displayed in scattergrams and histograms and exported.
APP Category 3
IF GLIAL CELLS
The IF Glial Cells APP allows the detection of astrocytes and microglia based on specific IF staining. The measurements assessed by the APP include the number of astrocytes and microglia and the number of branches of each cell (short attached and long attached).
APP Category 3
IF SKIN MORPHOLOGY
The IF Skin Morphology APP provides tissue detection including segmentation into epidermis and dermis based on specific IF staining. It segments the cells into nucleus, and/or perinuclear area and/or cytoplasm and determines the cellular phenotype of specific IF stained cell populations. The detected cells can be classified and visualized as being either within or outside of detected structures (epidermis and dermis). Each segmented cell compartment is measured for up to 20 intensity, statistics and morphometric parameters which can be displayed in diagrams and exported.
APP Category 3
IF PYKNOTIC NUCLEI
The IF Pyknotic Nuclei APP provides tissue detection and cell segmentation in combination with the detection of pyknotic nuclei (defined as completely condensed, round, high intensity nuclei) based on nuclei staining. Additionally, the APP allows to determine the cellular phenotype of specific IF stained cell populations as well as dot detection. It segments the detected cells into nucleus, and/or perinuclear area and/or cytoplasm. As outcome the APP provides parameters such as number, mean intensity and percentage of specific cell populations (including cells containing pyknotic nuclei), as well as dot parameters per segmented cell including count, mean intensity, total dot area, and sum of intensity as well as area and intensity list for all single dots.
APP Category 3
IF SYNAPTIC PUNCTA
The IF Synaptic Puncta APP allows the detection of nerve cells combined with the detection of synaptic puncta (dot-like synapse associated structures) based on specific IF markers (number technically unlimited). The measurements provided by the APP are number of detected puncta per cell and marker.
APP Category 2
IF SKELETAL MUSCLE
The IF Skeletal Muscle APP allows the segmentation of skeletal muscle tissue sections into muscle fibres and connective tissue based on specific IF staining. As outcome parameters are provided such as number of muscle fibres, area of the total tissue, muscle fibres and connective tissue.
Image: Courtesy of Stefania Petrini, Bambino Gesù Children’s Hospital, Rome
APP Category 2
IF GLOMERULI
The IF Glomeruli APP provides the detection of tissue, cells and the detection of glomeruli (stained by a specific marker). It segments the cells into nucleus, and/or perinuclear area and/or cytoplasm and determines the cellular phenotype of specific IF stained cell populations. The APP measures the distance of detected cellular objects to the glomeruli boundary (within and/or outside). The detected cells can be classified and visualized as being either within or outside (distance ranges can be defined) of the glomeruli. Each segmented cell compartment is measured for up to 20 intensity, statistics and morphometric parameters which can be displayed in diagrams and exported.
APP Category 3
