• Compact stage for inverted microscope operation

  • -190°C to 250°C with resistive heating and liquid nitrogen cooling option

  • 23 mm × 28 mm sample area

  • Sealed chamber with gas purging


Instec’s HCS321Gi hot and cold stage is designed specifically for inverted optical microscopes. Its small form factor allows for easy mounting onto all common inverted microscope platforms. The HCS321Gi offers the widest temperature range available for inverted microscope applications and a fully enclosed sample chamber for enhanced temperature uniformity.

Thermal Features

Temperature Control

mK2000 with programmable precision switching PID method

Sample Thermal Cover

Removable inner sample cover with additional window

Temperature Minimum

-80°C (with optional liquid N2 cooling)

-190°C (optional)

Temperature Maximum


Temperature Sensor

100 Ω Platinum RTD

Maximum Heating Rate

+80°C per minute at 100°C

Maximum Cooling Rate

-30°C per minute at 100°C

Minimum Heating and Cooling Rate

±0.01°C per minute

Temperature Resolution


Temperature Stability

±0.05°C (>25°C), ±0.1°C (<25°C)

Power Supply

Universal power input


Windows software to record and export temperature-time data

Optical Features

Optical Access

Reflection and transmission capability

Optical Windows

Removable and exchangeable windows permit full-spectrum transparency

Minimum Objective Working Distance

5.39 mm

Minimum Condenser Working Distance

11.21 mm

Top Window

27 mm diameter

Top Viewing Angle

±40° from normal

Transmission Aperture

5 mm diameter

Bottom Window

27 mm diameter

Bottom Viewing Angle

±50° from normal

Window Defrost

Integrated external window defrost

Structural Features

Sample Area

23 mm x 28 mm

Chamber Height

10.2 mm without removeable inner cover

5.2 mm with removeable inner cover

Frame Cooling

Integrated frame cooling with optional chiller system


Horizontal and vertical mounting capability

Frame Dimensions

135 mm x 134 mm x 23.5 mm


610 g

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